The test is bases qPCR (Quantitative Polymerase Chain Reaction) a technique used to identify microbial material, including bacteria, viruses and fungi. The technique was first developed in the early 1980s and became widely used in microbiological research in the 2000s.
Using qPCR also makes it possible to accurately determine the species of mould that are present. This allows analysts to distinguish between species that are naturally present in the outdoor air and species that only grow in damp indoor environments. In other words, accurately identifying mould species makes it possible to distinguish between problematic mould growth and mould more generally.